Voronezh, Voronezh, Russian Federation
Russian Federation
Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Information on the genetic structure of populations of forest tree plants is the basis for assessing the genetic po-tential of the species. These studies are particularly relevant for economically valuable species and species occupying extensive areas, like larch. Accurate information about the genetic structure of populations, the level of their genetic variability, the nature of its distribution within the range allows us to designate measures aimed at preserving the genet-ic resources of the species for the use in the country's economy and reproduction. During the analysis of electrophoretic spectra of the products of amplification of six nuclear microsatellite loci 42 allelic variants have been identified. An estimation of the genetic polymorphism of the population structure of forest plantations of Sukachev’s larch from the seeds of the Sverdlovsk region by microsatellite analysis for 6 pairs of pri-mers is given.
molecular and genetic methods, Sukachev's larch, observed number of alleles, effective number of alleles
1. Deryuzhkin, R.I. Biologicheskie osnovy semenovodstva i kul'tury listvennicy v Central'noy leso-stepi [Tekst]: Dis. d-ra s.-h. nauk 06.560.- Voronezh, 1970. 1 - 30 s.
2. Larionova A.Ya. Geneticheskaya izmenchivost' listvennicy sibirskoy v Nizhnem Priangar'e [Tekst]/ A.Ya.Larionova, N.V. Yahneva (Oreshkova), N.A. Kuz'mina //Lesovedenie. - 2003. - № 4. - S. 17-22.
3. Merkur'eva, E.K. Genetika populyaciy [Tekst] / E.K.Merkur'eva, G.G.Skripchenko, A.V. Bakay, F.R. Ba-kay, I.I. Kochish, A.P. Hramov. - M., 2004. - S. 8 - 20
4. Molekulyarnaya genetika: ucheb.-metod. posobie [Tekst] / Pod red. S.V. Boronnikovoy. Perm. un-t. - Perm', 2007 - S. 9 -13.
5. Nechaeva, Yu.S. Izuchenie polimorfizma ISSR-markerov v prirodnyh i iskusstvennyh populyaciyah li-stvennicy [Tekst]/ Yu.S. Nechaeva, S.V. Boronnikova, R.R. Yusupov, B. Haynce // Fundamental'nye issledova-niya. - 2013. - № 6-6. - S. 1426-1431.
6. Oreshkova, N.V. Allozimnyy polimorfizm listvennicy sibirskoy (Larix sibirica Le-deb.) [Tekst] / N.V. Oreshkova, A.Ya. Larionova // Lesnye ekosistemy Severo-Vostochnoy Azii i ih dinamika: mat-ly mezhdu-nar. konf. - Vladivostok, 2006. - S. 220 - 223.
7. Oreshkova, N.V. Geneticheskoe raznoobrazie listvennicy sibirskoy v Uzhurskoy lesostepi (Krasno-yarskiy kray) [Tekst] / N.V.Oreshkova, A.Ya.Larionova // Vestnik SVNC DVO RAN. - 2007. - № 3. - S. 50 - 55.
8. Oreshkova, N.V. Geneticheskie osobennosti i morfologicheskaya izmenchivost' listven-nicy sibirskoy v Altae-Sayanskoy gornoy oblasti [Tekst] / N.V.Oreshkova, A.P. Barchenkov // Vestnik KrasGAU. - 2010. - № 10. - S. 59 - 64.
9. Padutov, V. E. Metody molekulyarno-geneticheskogo analiza [Tekst] / V. E. Padutov, O. Yu. Baranov, E. V. Voropaev. - M. : Yunipol, 2007. - 176 s.
10. Programma i Metodika po punktu 59. Plan meropriyatiy («dorozhnoy karty») «Razvitie biotehnolo-giy i gennoy inzhenerii», utverzhdennogo rasporyazheniem Pravitel'stva RF Prirodopol'zovanie ot 18 iyulya 2013 g. № 1247-r. Programma i Metodika utverzhdena FBU «Roslesozaschita» ot 12.02.2014. [Tekst]. - Pushkino, 205 s.
11. Semerikov, V.L. Izuchenie geneticheskoy izmenchivosti listvennicy sibirskoy (Larix sibirica Ldb.) po izofermentnym lokusam [Tekst] / V.L. Semerikov, A.V. Matveev // Genetika. - 1995. - T. 31. - № 8. - S. 1107-1113.
12. Hedrik, F. Genetika populyaciy / F. Hedrik // Tehnosfera. - 2003 - 1-592 s.
13. Chalmers, K.J. Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers [Article] / K.J. Chalmers, R. Waugh, J.I. Sprent et al. // Heredity, 1992. V. 69. P. - 465-472.
14. Kalendar R. IRAP and REMAP: two new retrotransposon-based DNK fingerprinting techniques [Article] / R. Kalendar, T. Grob, M. Regina, F. Suoniemi, A. Schulman // Theor. Appl. Genet., 1999. V. 98. P.704-711.
15. Kalendar, R., Schulman, A.H. IRAP and REMAP for retrotransposon-based genotyping and fingerprinting [Article] / R. Kalendar, A.H. Schulman // Nature Protocols., 2006. №.1(5). - P. 2478 - 2484.
16. Krutovsky, K.V. et al. Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) mega gametophytes for whole genome de novo sequencing [Article] / KV Krutovsky, I.N. Tretyakova, N.V. Oreshkova, M.E. Pak, O.V. Kvitko, et al // In Vitro Cell Dev Biol Plant 50(5)., 2014. - 655 - 664.
17. Sambrook, J. Gel electrophoresis of DNA [Article] / J. Sambrook, E.F. Fritsh, and Manias, T. (Eds). // Mo-lecular Cloning: a Laboratory Manual. New York : Could Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1989. chapter 6.
18. Semerikov, V.L. Intra- and interspecific allozyme variability in Eurasian Larix Mill. species [Article] / V.L. Semerikov, L.F. Semerikov, M. Lascoux // Heredity. - 1999. - Vol. 82. - P. 193-204.