employee
Voronezh, Voronezh, Russian Federation
Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Dannaya rabota posvyaschena ocenke vliyaniya holodovogo stressa na fiziologicheskie parametry i geneticheskiy apparat selekcionno-cennyh sortov topolya. V hode issledovaniya provodilas' ocenka dinamiki raspuskaniya pochek i opredelenie ekspressii gena DREB2 u obrazcov razlichnyh sortov topolya, podvergnutyh iskusstvennomu promorazhivaniyu. Metodologiya postanovki opyta vklyuchala modelirovanie holodovogo stressa pri pomoschi nizkotemperaturnoy morozil'noy kamery, laboratornoe otraschivanie kontrol'nyh i promorozhennyh cherenkov v usloviyah iskusstvennogo osvescheniya. V dal'neyshem osuschestvlyalas' registraciya ontogeneticheskoy dinamiki raspuskaniya pochek. Pri sravnenii intensivnosti otrastaniya opytnyh i kontrol'nyh obrazcov vyyavleno, chto u nekotoryh sortov (‘Veduga’, ‘Bolid’) podvergnutye promorozke cherenki demonstriruyut bolee intensivnoe raspuskanie pochek, v to vremya kak drugie genotipy (‘Ivanteevskiy’, ‘ED-120’, ‘Kitayskiy’) proyavili otstavanie razlichnoy stepeni v skorosti raspuskaniya pochek promorozhennyh obrazov ot kontrol'nyh. Po rezul'tatam fenologicheskih nablyudeniy byli otobrany predstaviteli s kontrastnymi pokazatelyami (‘Sakrau’, ‘E.c.-38’ i ‘Volosistoplodnyy’) i podvergnuty dal'neyshemu izucheniyu na molekulyarno-geneticheskom urovne. Analiz pokazal povyshenie ekspressii gena DREB2 u dvuh opytnyh obrazcov sortov topolya (‘Volosistoplodnyy’ i ‘Sakrau’) posle vozdeystviya otricatel'nyh temperatur otnositel'no kontrol'nyh obrazcov. Promorazhivanie opytnyh rasteniy topolya ‘E.c.-38’ demonstrirovalo obratnyy rezul'tat. V celom mozhno zaklyuchit', chto nablyudenie dinamiki raspuskaniya pochek i ocenka ekspressii genov yavlyaetsya dostatochno informativnym markerom dlya izucheniya vliyaniya holodovogo stressa, odnako dlya utochneniya svyazi fenologicheskih parametrov s ekspressiey genov stressoustoychivosti trebuyutsya dopolnitel'nye issledovaniya.
topol', stress, promorazhivanie, ekspressiya, DREB2
1. Charu, L. Role of DREBs in regulation of abiotic stress responses in plants [Text] / L.Charu, M. Prasad// Journal of Experimental Botany. - 2011. - Vol. 62. - No. 14. - pp. 4731-4748,
2. Chen, J. Expression profiling and functional characterization of a DREB2-type gene from Populus euphratica[Text] / J. Chen, X. Xi, W. Yin // Biochemical and Biophysical Research Communications. - 2009. - Vol. 378. - pp. 483-487.
3. An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis[Text] / J. S. Kim [et al.] // Plant Cell Physiol. - 2011. - No. 52 (12). - pp. 2136-2146.
4. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis[Text] / Q. Liu [et al.] // Plant Cell. - 1998. - No. 10(8). - pp. 1391-1406.
5. Cloning and stress response analysis of the PeDREB2A and PeDREB1A genes in moso bamboo (Phyllostachys edulis) [Text] / H. L. Wu [et al.] // Genetics and Molecular Research. - 2015. - No. 14 (3). - pp. 10206-10223.
6. The gene family of dehydration responsive element-binding transcription factors in grape (Vitis vinifera): genome-wide identification and analysis, expression profiles, and involvement in abiotic stress resistance [Text] / T. Zhao, H. Xia, J. Liu,F. Ma // Mol. Biol. Rep. - 2013.
7. Besschetnov, P. P. Topol' (kul'tura i selekciya) [Tekst] / P. P. Besschetnov. - Alma-Ata, 1969. - 156 s.
8. Eprincev, A. T. Identifikaciya i issledovanie ekspressii genov [Tekst] : ucheb.-metod. posobie / A. T. Eprincev, V. N. Popov, D. N. Fedorin. - Voronezh, 2008. - S. 3-15.
9. Lihovskoy, V. V. Morozoustoychivost' krymskih aborigennyh sortov vinograda i ih gibridov [Tekst] / V. V. Lihovskoy // Nauchnyy zhurnal KubGAU. - 2016. - № 117(03). - 14 s.
10. Lyubimov, V. B. Tolerantnost' rasteniy k rezhimu deficita vlagi i temperaturnomu rezhimu [Tekst] / V. B. Lyubimov, N. P. Kotova // Vestnik Bryanskogo gosudarstvennogo universiteta. - 2010. - 6 s.
11. Pankova, E. I. O probleme ocenki zasolennosti pochv i metodike krupnomasshtabnogo cifrovogo kartografirovaniya zasolennyh pochv [Tekst] / E. I. Pankova, M. V. Konyushkova, I. N. Gorohova // Ekosistemy: ekologiya i dinamika. - 2017. - T. 1. - № 1. - S. 26-54.
12. Carev, A. P. Sortovedenie topolya [Tekst] / A. P. Carev. - Voronezh, 1986. - 152 s.
